Spectrum of Methods
Pick Your Method from Our Large Portfolio
Method and Application
- Compendial methods, e.g. visual inspection, pH, conductivity
According to Ph. Eur./USP
- SDS-PAGE
Reducing, non-reducing, or native Coomassie, silver, or fluorescence staining or immunodetection for identity or purity testing
- Isoelectric focusing
- Capillary electrophoresis
Determination of purity by CE-SDS for proteins or laser-induced fluorescence detection for pDNA. Determination of residual TFA
- Protein quantification
Microtiter plate and cuvette based assays such as BCA™, Bradford, and NanoOrange®
- UV absorption
Measurement at A280 and A260 for content determination or scanning for characterization
- HPLC/UPLC
Application with UV, DAD, IR, or light-scattering detection:
- Reverse phase (RP-HPLC)
- Size exclusion (SEC)
- Affinity chromatography
- Ion exchange chromatography
- LC-MS
Determination of intact mass for proteins (Identification)
Peptid Mapping for verification primary structure
Peptide Mapping for identification modifications (e.g. oxidation, deamidation)
- Peptide map
Protein digestion with HPLC separation and UV detection
- Quantification of residual DNA
- Threshold system of molecular devices
- Quantitative PCR
- Quantification of host cell proteins
- Development and characterization of mock cell line and immunization substrate
- Generation and characterization of antibodies (immunization and purification in combination)
- Development of HCP assay in ILA or ELISA format
- ELISA
Application for protein concentration or impurity testing (e.g. process related impurities)
- Agarose gel electrophoresis
With ethidium bromide or fluorescence staining for DNA and RNA
- Restriction analysis
Enzymatic digestion and agarose gel electrophoresis for identity verification
- Quantitative PCR used for:
- Determining residual genomic DNA
- Determining copy number by combined Q-PCR methods
- Quantification of RNA and DNA
Fluorescence-based microtiter assays (e.g. PicoGreen®, RiboGreen®)
- E.coli strain characterization
Phenotypic by API and genotypic by RAPD
- Replica Picking
For determination of plasmid-retaining cells
- Activity assays
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Analytical development at Richter BioLogics is set up to establish state-of-the-art, fully compliant solutions for your molecule.
